1. Positive Amylase Test = has a clearing
tests for exoenzyme amylase which hydrolyzes starch (a polysaccharide)
outside of cell into glucose subunits which can enter cell
growth medium= starch agar
reagent= iodine- binds to starch
ie #8= positive; #16=negative
2. Positive Lipase Test =has a clearing
tests for exoenzyme lipase which hydrolyzes tributyrin (a triglyceride) into
glycerol and 3 fatty acid subunits which can be taken into cell for energy
growth medium= tributyrin agar -has lipid so it looks white
no reagent
ie. #8 positive; # 16 negative
3. Positive Gelatinase Test= has a clearing
tests for exoenzyme gelatinase which hydrolyzes gelatin (a protein) into
amino acids which can enter cell and be used in metabolism
growth medium= gelatin agar
no reagent
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