Fermentation Of Carbohydrates Test
tests for Fermentation enzymes
Mediums= glucose (G), Lactose (L), or Sucrose (S)
all tubes contain protein and pH indicator
(acid= yellow; neutral=green; base= blue)
protein sparring- if bacteria can't use carbohydrates for metabolism,
it will use protein which causes the medium to turn blue
Positive Result= if tubes are: yellow +/- bubble
Key Symbols to Know for Test
A= Acid= yellow color
AG= Acid + Gas= yellow and air bubble
K= alkaline= blue color
NC= no change
A= Acid= yellow color (ignore middle tube)= Positive Result
AG= Acid + Gas= yellow and air bubble= Positive Result
K= alkaline= blue color= Negative Result, and Protein Sparring
NC= no change= Negative Result
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