1. Hydrogen Sulfide (H2S) Test/Indole Test/ Motility (SIM)
a. Hydrogen Sulfide (H2S) Test
b. Indole Test
c. Motility Test
2. Phenylalanine Deaminase Test
3. Rapid Ornithine Decarboxylase Test
4. Urease Test
1. Hydrogen Sulfide (H2S) Test/Indole Test/ Motility (SIM)
a. Hydrogen Sulfide (H2S) Test
-tests for enzyme cysteine desulfurase-bacteria with this enzyme can break down amino acid cysteine
- growth medium- SIM medium agar
Positive= black
Negative= medium remains yellow or black line forms just below surface
Hydrogen Sulfide (H2S) Test
Positive=black
Neg= yellow or yellow with black line
Hydrogen Sulfide (H2S) Test
b. Indole Test
-tests for enzyme tryptophanase- if bacteria has they can break down tryptophan
- growth medium=SIM medium
- Positive= pink strip
-Negative= white strip (no change)
Indole Test
-note #22 (the middle strip) is the ONLY NEGATIVE =WHITE strip
Indole Test
Positive=pink
Indole test
positive-= pink
Indole Test
#22= negative= white
#27= positive= pink
c.Motility Test
+= black or yellow with fuzzy growth
- = yellow with 2d ribbon growth
*note-when reading motility- ignore the little black line just below surface?
Motility Test=
Negative=2d Ribbon
Motility Test
Negative= 2D ribbon
Motility Test
Positive= Black
Motility Test
Positive= Yellow and Fuzzy
(think pipe cleaner)
Motility Test
Positive= Yellow and Fuzzy
2. Phenylalanine Deaminase Test
-tests for enzyme phenylalanine deaminase
-growth medium= phenylalanine agar
-reagent= ferric chloride (FeCl3)
-after incubating, the reagent ferric chloride is added to the medium
+ test= green = -phenylpyruvic acid reacts with ferric chloride and turns green
- test= no change- medium remains yellow
Phenylalanine Deaminase Test
Phenylalanine Deaminase Test
Positive= green
Phenylalanine Deaminase Test
Negative= yellow= no color change
3. Rapid Ornithine Decarboxylase Test
-tests for enzyme ornithine decarboxylase
- removes carboxyl group from amino acid ornithine
-growth medium= rapid ornithine medium
Positive test= purple
Negative test= grey/clear
Rapid Ornithine Decarboxylase Test
#32= negative= grey/clear
#16= positive=purple
4. Urease Test
test for enzyme urease
growth medium= urea agar
Positive test= fushia= presence of urease causes the formation of basic products ammonia and ammonium hydroxide
Negative test= medium remains yellow= medium stays acidic
No comments:
Post a Comment