Lab 31: Streptococcus pyogenes Rapid Test
1. Rapid tests= immunological assays that use Ag-Ab reactions to identify a specific organism or agent
-tests give results within minutes and can be run in hospital labs/dr.s offices
In this lab, we see a demonstration of Streptococcus pyogenes modified direct ELISA test (“sandwich assay”= Ab-Ag-Ab)
2. Modified DIrect Elisa Test
-uses a paper stip that has an area embedded with anti-Strep antibodies to detect S. pyogenes in a sample
- samples are taken from Beta hemolytic colonies cultured from throat swab grown on sheep’s blood agar plates
How Test Works-
1. Sample with possible S. pyogenes is added to test tube which contains anti-Strep antibodies conjugated with a blue dye
-if S. pyogenes is present they bind to anti-Strep antibodies
2. A paper strip is then placed into the tube and Ag-Ab complexes wick up strip
3. Area 1= contains anti-Strep antibodies attached to the strip
-as the fluid wicks up the strip, if Strep Ag---anti-Strep antibody complexes are in it, the Strep Ag will bind to the anti-Strep antibodies attached to the strip
-ie. it forms an Ab-Ag-Ab with blue dye
-the blue dye causes a blue line to form in this area
+reaction= blue strip and pink line
- reaction= just pink line
4. Area 2=Pink line= as the fluid continues to wick up the strip, a pink line confirms that the test is functioning properly
ie. the fluid wicked past area 1 with attached antibodies
-so if no lines appear or just blue line-something is wrong with test
Positive Negative
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